Stress: Plant Protein Database
        PSPDB: Plant Stress Protein Database
Searching PSPDB database Content 

STEP1: The Search Menu offers six catalogues
The database can be browsed or searched by using the search options available in each of the respective categories, namely, taxonomy, species, keyword, family and citation. Multiple search terms can be selected in each of these categories except citation.

General Rules
Use the available search terms to search the database. Select a search term and click the ‘query button’. Alternatively, use multiple search terms by holding down the shift key [on your keyboard], make your selections and click the ‘query button’. Click the ‘reset button’ to clear selections For each plant protein entry there are a common set of tools. The user does not necessarily have to go the tools page for the following tools: BLAST, ClustalW, HMMER. These tools are embedded in each plant protein entry and can be used by clicking the respective ‘Tool’ buttons.


Taxonomy
Select a taxonomy category and click the query button. All records for that query will be displayed. To select multiple taxonomies hold down the shift key [on your keyboard] and make your selections. The amount in brackets indicates the minimum number of PSPDB peptide entries for that taxonomy category. Click the ‘reset button’ to clear selections.
Species
Select a species category and click the query button. All records for that species will be displayed. To select multiple species hold down the shift key [on your keyboard] and make your selections. The amount in brackets indicates the minimum number of PSPDB peptide entries for that taxonomy category. Click the ‘reset button’ to clear selections
Citation
Browse entries using article title, journal name, author or publication year. The user can view contributions to PSPDB peptide information via the journal, author and papers that they were originally sourced from. For instance, select an article title and click the ‘query button’ . The peptide record for that article title will be shown. Select an author and all peptide records related to the author will be shown. The amount in brackets indicates the number of contributions from that source. Selection of multiple search terms cannot be used in this category.
Keyword
Select a keyword and click the query button. All records for that family will be displayed. To select multiple keywords hold down the shift key [on your keyboard] and make your selections. The amount in brackets indicates the minimum number of PSPDB peptide entries for that taxonomy category. Click the ‘reset button’ to clear selections
Families
Select a family and click the query button. All records for that family will be displayed. To select multiple species hold down the shift key [on your keyboard] and make your selections. The amount in brackets indicates the minimum number of PSPDB peptide entries for that taxonomy category. Click the ‘reset button’ to clear selections
Advance Search
Select a category and click the ‘query button’. All entries in that category will be displayed. For instance, if you select protein and click the ‘query button’ all protein records in the database will be displayed. Alternatively, select a category and restrict the results by adding a search term [in the search box]. For instance, if you select taxonomy and enter ‘Insecta’ in the ‘search box’. All proteins from the Insecta taxonomy will be displayed. Click the ‘reset button’ to clear the search box


STEP2: Select one/multiple entry/entries

Select your search term/s and click the ‘query button’.


STEP3: Viewing Short view of peptides

The results are displayed, showing a minimum amount of information for each plant protein entry. Click on the ‘View Seq[Fasta] button’ to view all the sequence in FASTA format. Click on ‘BLAST button’ to have all sequence(s) blasted through BLAST and results are shown. Click on ‘ClustalW button’ to have all the multiple sequences (more than three) to be analysed thruogh ClustalW and Phylogenic and results are shown. Click on ‘Hmmer button’ to have all sequences analysed through Hmmer profiles and results are shown.


STEP4: Viewing plant protein entries in complete format

Each plant protein entries are shown with complete information on a segmented basis as Entry Information, Name and Origin, Bibliography, Comments, Cross References (where 200+ databases are linked to our PSPDB), Keywords (as given by UniProtKB), Classification by PSPDB (Biotic/Abiotic, Stress Factors), Sequence Details. For the sake of space, we have shown a part of the image on a complete format entry here with.